Supramolecular assemblies, formed through non-covalent interactions, has become particularly attractive to develop dynamic and responsive architectures to address living systems at the nanoscale. Cucurbituril (CB), a pumpkin shaped macrocylic host molecule, has been successfully used to construct various self-assembled architectures for biomedical applications since it can simultaneously bind two aromatic guest molecules within its cavity. Such architectures can also be designed to respond to external stimuli. Integrating living organisms as an active component into such supramolecular architectures would add a new dimension to the capabilities of such systems. To acheive this, we have incorporated supramolecular functionality at the bacterial surface by genetically modifying a transmembrane protein to display a CB-binding motif as part of a cystine-stabilized miniprotein. We were able to confirm that this supramolecular motif on the bacterial surface specifically binds CB and forms multiple intercellular ternary complexes leading to aggregation of the bacterial solution. We performed various aggregation experiments to understand how CB interacts with this bacterial strain and also demonstrate that it can be chemically reversed using a competitor. To confirm that this strain can be incorporated with a CB based architecture, we show that the bacterial cells were able to adhere to CB SAMs on gold and still retain considerable motility for several hours, indicating that the system can potentially be used to develop supramolecular bacterial biomotors. The bacterial strain also has the potential to be combined with other CB based architectures like nanoparticles, vesicles and hydrogels.
Article by Jordi Cabanas-Danés has been selected as a Most Accessed Article for 2014 in Journal of Materials Chemistry B
The article "Chemical strategies for the presentation and delivery of growth factors" by Jordi Cabanas-Danés has been selected by the Journal of Materials Chemistry B, as a Most Accessed Article for 2014 and as such it has been included in the Most Accessed Articles 2014 web collection for the Journal which can be viewed here
As the number one cause of death globally, cardiovascular diseases represent an enormous burden to public health. One of the most common life-threatening problems is the abnormal narrowing of the coronary artery, which restricts blood flow and can eventually lead to a heart attack. In order to prevent this, coronary artery stenting is a frequently performed clinical procedure involving the implantation of a small mesh tube that supports the inner walls of the artery. Although this intervention saves countless lives every year, it is not without problems…